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After 2 days in vitro (DIV), the cultures were changed to Prenatal Mouse Neuron Media (PMNM) composed of Neurobasal media (Gibco/Fisher Scientific, Cat# 21-103-049) supplemented with B-27 Plus (Fisher Scientific, Cat# A3582801), GlutaMAX Supplement, and penicillin/streptomycin

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Potential risks including bruising, vein irritation, fluid overload, or rare allergic reactions are discussed during consultation, and suitability is confirmed before treatment

10.1002/ps.2780450317 57 GullnerG.KomivesT

Based on the functional differences, CD can be divided into accidental cell death (ACD) and regulated cell death (RCD)